Detailed information of TA system TA180661

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101555..101808
• Replicon: plasmid pJX4-2
• Accession: NZ_CP064237
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IS499_RS28790
• Protein ID: WP_001312851.1
• Coordinates: 101555..101704 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	101749..101808 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS499_RS28755	96914..97329	-	416	Protein_120	IS1 family transposase	-
IS499_RS28760	97578..97979	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IS499_RS28765	97912..98169	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IS499_RS28770	98262..98915	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IS499_RS28775	99854..100711	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IS499_RS28780	100704..100778	-	75	WP_001375168.1	RepA leader peptide Tap	-
IS499_RS28785	101023..101271	-	249	WP_000083837.1	replication regulatory protein RepA	-
IS499_RS28790	101555..101704	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	101749..101808	+	60	NuclAT_1	-	Antitoxin
-	101749..101808	+	60	NuclAT_1	-	Antitoxin
-	101749..101808	+	60	NuclAT_1	-	Antitoxin
-	101749..101808	+	60	NuclAT_1	-	Antitoxin
IS499_RS28795	102009..102239	-	231	WP_001736714.1	hypothetical protein	-
IS499_RS28800	102403..103002	-	600	WP_032083981.1	hypothetical protein	-
IS499_RS28805	103388..103588	-	201	WP_015059022.1	hypothetical protein	-
IS499_RS28810	103720..104280	-	561	WP_000139328.1	fertility inhibition protein FinO	-
IS499_RS28815	104335..105081	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IS499_RS28820	105101..105262	-	162	Protein_133	DNA helicase	-
IS499_RS28825	105326..106030	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IS499_RS28830	106082..106426	+	345	Protein_135	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	cmlA1 / blaCTX-M-14 / blaSHV-12 / blaKPC-2 / blaTEM-1B	-	1..165027	165027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T180661 WP_001312851.1 NZ_CP064237:c101704-101555 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T180661 NZ_CP085627:2057575-2057853 [Erwinia rhapontici]
ATGATTAAAAGCTTTAAGCATAAGGGACTAAAAAAATTCTTTCTAAAAGATGATAGTAGCGGTATCGAACAAAAGCTGGT
GGGTCGAATAAGGAACCGCCTGACCGTGATTGATGCGGCAGAAAAAATCGGAGCGATCGATATGCCTGGTTACGACCTGC
ATCCGCTCAAAGGTGATCGGCAGGAAATGTGGTCAGTTAGCGTATCGGGTAACTGGCGTATCACGTTTACGTTTATTGAT
GGCGATGCTGAGATTCTTAACCTGGAGGATTACCACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT180661 NZ_CP064237:101749-101808 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin