Detailed information of TA system TA180472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 97700..98370
• Replicon: plasmid pJX12-1
• Accession: NZ_CP064206
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-12

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IS504_RS26800
• Protein ID: WP_004213072.1
• Coordinates: 97927..98370 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IS504_RS26795
• Protein ID: WP_004213073.1
• Coordinates: 97700..97930 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS504_RS26770	93923..94822	-	900	WP_004225022.1	nucleotide-binding protein	-
IS504_RS26775	94812..95102	-	291	WP_004213078.1	hypothetical protein	-
IS504_RS26780	95454..95660	+	207	WP_004213077.1	hypothetical protein	-
IS504_RS26785	95650..95943	-	294	WP_004213076.1	hypothetical protein	-
IS504_RS26790	95959..97092	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IS504_RS26795	97700..97930	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS504_RS26800	97927..98370	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS504_RS26805	98384..98800	-	417	Protein_103	restriction endonuclease	-
IS504_RS26810	98804..99097	-	294	Protein_104	transposase	-
IS504_RS26815	99514..100149	+	636	Protein_105	response regulator	-
IS504_RS26820	100664..101070	-	407	Protein_106	GAF domain-containing protein	-
IS504_RS26825	101161..102081	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
IS504_RS26830	102130..102621	-	492	WP_011251327.1	DM13 domain-containing protein	-
IS504_RS26835	102684..102959	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..230835	230835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T180472 WP_004213072.1 NZ_CP064206:97927-98370 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T180472 NZ_CP085468:c2230880-2230629 [Aeromonas caviae]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT180472 WP_004213073.1 NZ_CP064206:97700-97930 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT180472 NZ_CP085468:c2230632-2230378 [Aeromonas caviae]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620