Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3847372..3847997 Replicon chromosome
Accession NZ_CP064129
Organism Klebsiella pneumoniae strain M911-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IOD52_RS18900 Protein ID WP_002882817.1
Coordinates 3847614..3847997 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD52_RS18895 Protein ID WP_004150355.1
Coordinates 3847372..3847614 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD52_RS18870 3843363..3843962 + 600 WP_004151865.1 glucose-1-phosphatase -
IOD52_RS18875 3843956..3844816 + 861 WP_004146232.1 virulence factor BrkB family protein -
IOD52_RS18880 3844813..3845250 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD52_RS18885 3845295..3846236 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IOD52_RS18890 3846250..3847167 - 918 WP_015959199.1 alpha/beta hydrolase -
IOD52_RS18895 3847372..3847614 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD52_RS18900 3847614..3847997 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD52_RS18905 3848171..3849100 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD52_RS18910 3849097..3849732 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD52_RS18915 3849729..3850631 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T180363 WP_002882817.1 NZ_CP064129:3847614-3847997 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T180363 NZ_CP085190:78399-78815 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT180363 WP_004150355.1 NZ_CP064129:3847372-3847614 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT180363 NZ_CP085190:78172-78402 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References