Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1070243..1070897 Replicon chromosome
Accession NZ_CP064103
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ISU84_RS05155 Protein ID WP_000244781.1
Coordinates 1070490..1070897 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ISU84_RS05150 Protein ID WP_000354046.1
Coordinates 1070243..1070509 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS05130 1066321..1067754 - 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -
ISU84_RS05135 1067799..1068110 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
ISU84_RS05140 1068274..1068933 + 660 WP_000250269.1 hemolysin III family protein -
ISU84_RS05145 1069010..1069990 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
ISU84_RS05150 1070243..1070509 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ISU84_RS05155 1070490..1070897 + 408 WP_000244781.1 protein YgfX Toxin
ISU84_RS05160 1070937..1071458 - 522 WP_001055874.1 flavodoxin FldB -
ISU84_RS05165 1071570..1072466 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ISU84_RS05170 1072491..1073201 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISU84_RS05175 1073207..1074940 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T180127 WP_000244781.1 NZ_CP064103:1070490-1070897 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T180127 NZ_CP085069:2817746-2817853 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT180127 WP_000354046.1 NZ_CP064103:1070243-1070509 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT180127 NZ_CP085069:c2817693-2817632 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References