Detailed information of TA system TA179913

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4419468..4420303
• Replicon: chromosome
• Accession: NZ_CP063983
• Organism: Escherichia coli strain 25DN

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: IR210_RS21180
• Protein ID: WP_000854759.1
• Coordinates: 4419468..4419845 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IR210_RS21185
• Protein ID: WP_194446068.1
• Coordinates: 4419935..4420303 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR210_RS21155	4415579..4417219	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
IR210_RS21160	4417992..4418168	-	177	Protein_4148	helix-turn-helix domain-containing protein	-
IR210_RS21165	4418532..4418690	-	159	WP_001467148.1	hypothetical protein	-
IR210_RS21170	4418790..4418966	-	177	WP_000839286.1	DUF957 domain-containing protein	-
IR210_RS21175	4418983..4419471	-	489	WP_000761690.1	hypothetical protein	-
IR210_RS21180	4419468..4419845	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
IR210_RS21185	4419935..4420303	-	369	WP_194446068.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IR210_RS21190	4420466..4420687	-	222	WP_000692345.1	DUF987 domain-containing protein	-
IR210_RS21195	4420750..4421226	-	477	WP_001186775.1	RadC family protein	-
IR210_RS21200	4421242..4421715	-	474	WP_001350782.1	antirestriction protein	-
IR210_RS21205	4422057..4422875	-	819	WP_001234738.1	DUF945 domain-containing protein	-
IR210_RS21210	4423030..4423188	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4407928..4434829	26901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T179913 WP_000854759.1 NZ_CP063983:c4419845-4419468 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T179913 NZ_CP084899:252301-252699 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13463.24 Da
• Isoelectric Point: 6.6255

>AT179913 WP_194446068.1 NZ_CP063983:c4420303-4419935 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWGGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT179913 NZ_CP084899:252005-252298 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6