Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4523378..4524024 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name higB Uniprot ID A0A2V3KK24
Locus tag IOD50_RS21855 Protein ID WP_032731351.1
Coordinates 4523677..4524024 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag IOD50_RS21850 Protein ID WP_008806992.1
Coordinates 4523378..4523677 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS21830 4519584..4520342 - 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -
IOD50_RS21835 4520392..4521222 - 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
IOD50_RS21840 4521273..4521602 - 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
IOD50_RS21845 4521807..4523315 + 1509 WP_032740278.1 glycerol-3-phosphate dehydrogenase -
IOD50_RS21850 4523378..4523677 - 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
IOD50_RS21855 4523677..4524024 - 348 WP_032731351.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IOD50_RS21860 4524239..4526686 - 2448 WP_008806990.1 glycogen phosphorylase -
IOD50_RS21865 4526704..4528137 - 1434 WP_004202133.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13474.43 Da        Isoelectric Point: 6.2327

>T179841 WP_032731351.1 NZ_CP063954:c4524024-4523677 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKETITLADREFSQHLTKER

Download         Length: 348 bp

>T179841 NZ_CP084896:c1648978-1648691 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT179841 WP_008806992.1 NZ_CP063954:c4523677-4523378 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179841 NZ_CP084896:c1649217-1648978 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KK24

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References