Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 207285..207904 Replicon chromosome
Accession NZ_CP063953
Organism Klebsiella variicola strain S131-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISD12_RS00890 Protein ID WP_002892050.1
Coordinates 207686..207904 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISD12_RS00885 Protein ID WP_008805436.1
Coordinates 207285..207659 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD12_RS00875 202440..203633 + 1194 WP_043875731.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISD12_RS00880 203656..206802 + 3147 WP_064179935.1 multidrug efflux RND transporter permease subunit AcrB -
ISD12_RS00885 207285..207659 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISD12_RS00890 207686..207904 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISD12_RS00895 208063..208629 + 567 WP_008805435.1 maltose O-acetyltransferase -
ISD12_RS00900 208601..208729 - 129 Protein_178 hypothetical protein -
ISD12_RS00905 208766..209236 + 471 WP_008805434.1 YlaC family protein -
ISD12_RS00910 209205..210662 - 1458 WP_172749140.1 PLP-dependent aminotransferase family protein -
ISD12_RS00915 210763..211461 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISD12_RS00920 211458..211598 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISD12_RS00925 211598..211861 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179811 WP_002892050.1 NZ_CP063953:207686-207904 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179811 NZ_CP084895:c1414805-1414611 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179811 WP_008805436.1 NZ_CP063953:207285-207659 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179811 NZ_CP084895:1414434-1414612 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References