Detailed information of TA system TA179620

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25513..25767
• Replicon: plasmid pS183-1.2
• Accession: NZ_CP063929
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: ISA68_RS26235
• Protein ID: WP_015387399.1
• Coordinates: 25561..25767 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	25513..25574 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS26200	21165..21377	+	213	WP_005012601.1	hypothetical protein	-
ISA68_RS26205	21678..21767	-	90	Protein_28	IS1 family transposase	-
ISA68_RS26210	21822..22499	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
ISA68_RS26215	22499..22846	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
ISA68_RS26220	22866..24437	+	1572	WP_023149734.1	IS66 family transposase	-
ISA68_RS26225	24475..25091	-	617	Protein_32	IS1 family transposase	-
ISA68_RS26230	25192..25374	+	183	WP_000968309.1	hypothetical protein	-
-	25513..25574	-	62	NuclAT_3	-	Antitoxin
-	25513..25574	-	62	NuclAT_3	-	Antitoxin
-	25513..25574	-	62	NuclAT_3	-	Antitoxin
-	25513..25574	-	62	NuclAT_3	-	Antitoxin
ISA68_RS26235	25561..25767	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
ISA68_RS26240	26051..26308	+	258	WP_000083833.1	replication regulatory protein RepA	-
ISA68_RS26245	26544..26618	+	75	WP_032336874.1	RepA leader peptide Tap	-
ISA68_RS26250	26611..27056	+	446	Protein_37	incFII family plasmid replication initiator RepA	-
ISA68_RS26255	27002..27484	-	483	Protein_38	VENN motif pre-toxin domain-containing protein	-
ISA68_RS26260	28377..29597	+	1221	WP_000410951.1	arginine deiminase	-
ISA68_RS26265	29608..30519	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sitABCD / ARR-3 / catB3	iutA / iucD / iucC / iucB / iucA	1..190929	190929
	-	inside	Integron	-	iutA / iucD / iucC / iucB / iucA	364..190928	190564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T179620 WP_015387399.1 NZ_CP063929:25561-25767 [Klebsiella pneumoniae]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T179620 NZ_CP084859:c3946830-3946513 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT179620 NZ_CP063929:c25574-25513 [Klebsiella pneumoniae]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin