Detailed information of TA system TA179618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 132258..133009
• Replicon: plasmid pS183-1.1
• Accession: NZ_CP063928
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ISA68_RS25635
• Protein ID: WP_048333033.1
• Coordinates: 132258..132740 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: ISA68_RS25640
• Protein ID: WP_004902250.1
• Coordinates: 132731..133009 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS25615	128657..129304	-	648	WP_014386537.1	EcsC family protein	-
ISA68_RS25620	129331..130086	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
ISA68_RS25625	130187..130579	-	393	WP_032442757.1	hypothetical protein	-
ISA68_RS25630	130684..131223	-	540	WP_004902239.1	hypothetical protein	-
ISA68_RS25635	132258..132740	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
ISA68_RS25640	132731..133009	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
ISA68_RS25645	133131..133343	-	213	WP_016946198.1	hypothetical protein	-
ISA68_RS25650	133451..133792	-	342	WP_032437747.1	hypothetical protein	-
ISA68_RS25655	134698..135012	+	315	WP_023288299.1	hypothetical protein	-
ISA68_RS25660	135256..135738	-	483	WP_172694439.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 / rmpA / iroN / iroD / iroC / iroB	1..208376	208376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T179618 WP_048333033.1 NZ_CP063928:c132740-132258 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T179618 NZ_CP084859:1971203-1971305 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT179618 WP_004902250.1 NZ_CP063928:c133009-132731 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT179618 NZ_CP084859:c1971312-1971167 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3