Detailed information of TA system TA179557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4439552..4440149
• Replicon: chromosome
• Accession: NZ_CP063922
• Organism: Klebsiella pneumoniae strain BM338-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: ISA71_RS21585
• Protein ID: WP_004893639.1
• Coordinates: 4439552..4439869 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: ISA71_RS21590
• Protein ID: WP_004142561.1
• Coordinates: 4439862..4440149 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA71_RS21570	4435256..4436683	-	1428	WP_004176980.1	MFS transporter	-
ISA71_RS21575	4436792..4437658	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
ISA71_RS21580	4438324..4439367	+	1044	WP_004893645.1	DUF2157 domain-containing protein	-
ISA71_RS21585	4439552..4439869	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA71_RS21590	4439862..4440149	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA71_RS21595	4440414..4441067	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA71_RS21600	4441187..4441555	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
ISA71_RS21605	4441555..4442136	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
ISA71_RS21610	4442316..4443431	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
ISA71_RS21615	4443462..4443803	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA71_RS21620	4443821..4444069	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T179557 WP_004893639.1 NZ_CP063922:4439552-4439869 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179557 NZ_CP084829:c4629794-4629510 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT179557 WP_004142561.1 NZ_CP063922:4439862-4440149 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT179557 NZ_CP084829:c4630026-4629784 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1