Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2540487..2541133 Replicon chromosome
Accession NZ_CP063921
Organism Klebsiella variicola strain BM366-1

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag ISA73_RS11915 Protein ID WP_008806991.1
Coordinates 2540487..2540834 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag ISA73_RS11920 Protein ID WP_008806992.1
Coordinates 2540834..2541133 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA73_RS11905 2536374..2537807 + 1434 WP_004202133.1 glycogen synthase GlgA -
ISA73_RS11910 2537825..2540272 + 2448 WP_008806990.1 glycogen phosphorylase -
ISA73_RS11915 2540487..2540834 + 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA73_RS11920 2540834..2541133 + 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
ISA73_RS11925 2541196..2542704 - 1509 WP_008806993.1 glycerol-3-phosphate dehydrogenase -
ISA73_RS11930 2542908..2543237 + 330 WP_023291211.1 thiosulfate sulfurtransferase GlpE -
ISA73_RS11935 2543288..2544118 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
ISA73_RS11940 2544168..2544926 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T179528 WP_008806991.1 NZ_CP063921:2540487-2540834 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T179528 NZ_CP084823:848025-848408 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT179528 WP_008806992.1 NZ_CP063921:2540834-2541133 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179528 NZ_CP084823:847783-848025 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References