Detailed information of TA system TA179525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1794828..1796509
• Replicon: chromosome
• Accession: NZ_CP063921
• Organism: Klebsiella variicola strain BM366-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: ISA73_RS08505
• Protein ID: WP_032734038.1
• Coordinates: 1794828..1795745 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISA73_RS08510
• Protein ID: WP_032734037.1
• Coordinates: 1795751..1796509 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA73_RS08485	1790134..1791654	+	1521	WP_064324014.1	sugar ABC transporter ATP-binding protein	-
ISA73_RS08490	1791651..1792649	+	999	WP_004206363.1	ribose ABC transporter permease	-
ISA73_RS08495	1792680..1793621	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISA73_RS08500	1793696..1794703	+	1008	WP_022065799.1	carbohydrate kinase family protein	-
ISA73_RS08505	1794828..1795745	-	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA73_RS08510	1795751..1796509	-	759	WP_032734037.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA73_RS08515	1796594..1797322	-	729	WP_194387312.1	response regulator transcription factor	-
ISA73_RS08520	1797595..1799742	+	2148	WP_194387313.1	formate dehydrogenase subunit alpha	-
ISA73_RS08525	1800096..1800782	+	687	WP_016160120.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T179525 WP_032734038.1 NZ_CP063921:c1795745-1794828 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179525 NZ_CP084820:73244-73726 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGTAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCACAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28693.08 Da
• Isoelectric Point: 10.2152

>AT179525 WP_032734037.1 NZ_CP063921:c1796509-1795751 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179525 NZ_CP084820:72990-73256 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2