Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2190987..2191606 Replicon chromosome
Accession NZ_CP063916
Organism Klebsiella variicola strain BS327-2-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA76_RS10540 Protein ID WP_002892050.1
Coordinates 2190987..2191205 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA76_RS10545 Protein ID WP_008805436.1
Coordinates 2191232..2191606 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA76_RS10505 2187030..2187293 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA76_RS10510 2187293..2187433 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA76_RS10515 2187430..2188128 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA76_RS10520 2188229..2189686 + 1458 WP_194387274.1 PLP-dependent aminotransferase family protein -
ISA76_RS10525 2189655..2190125 - 471 WP_008805434.1 YlaC family protein -
ISA76_RS10530 2190162..2190290 + 129 Protein_2040 hypothetical protein -
ISA76_RS10535 2190262..2190828 - 567 WP_008805435.1 maltose O-acetyltransferase -
ISA76_RS10540 2190987..2191205 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA76_RS10545 2191232..2191606 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA76_RS10550 2192089..2195235 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA76_RS10555 2195258..2196451 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179472 WP_002892050.1 NZ_CP063916:c2191205-2190987 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179472 NZ_CP084776:8875-9357 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179472 WP_008805436.1 NZ_CP063916:c2191606-2191232 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179472 NZ_CP084776:8621-8887 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References