Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1723237..1723856 Replicon chromosome
Accession NZ_CP063912
Organism Klebsiella variicola strain BS359-2-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA79_RS08385 Protein ID WP_002892050.1
Coordinates 1723638..1723856 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA79_RS08380 Protein ID WP_008805436.1
Coordinates 1723237..1723611 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA79_RS08370 1718392..1719585 + 1194 WP_043875731.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISA79_RS08375 1719608..1722754 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA79_RS08380 1723237..1723611 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA79_RS08385 1723638..1723856 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA79_RS08390 1724015..1724581 + 567 WP_008805435.1 maltose O-acetyltransferase -
ISA79_RS08395 1724553..1724681 - 129 Protein_1658 hypothetical protein -
ISA79_RS08400 1724718..1725188 + 471 WP_008805434.1 YlaC family protein -
ISA79_RS08405 1725157..1726614 - 1458 WP_110202102.1 PLP-dependent aminotransferase family protein -
ISA79_RS08410 1726715..1727413 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA79_RS08415 1727410..1727550 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA79_RS08420 1727550..1727813 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179414 WP_002892050.1 NZ_CP063912:1723638-1723856 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179414 NZ_CP084741:1845719-1846033 [Pectobacterium carotovorum]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAACGTCGTTTATCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTTC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCATGCCTTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTTCTCAGGTAAAGGCGGCACTAGACTTCCTCATCGATGGGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179414 WP_008805436.1 NZ_CP063912:1723237-1723611 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179414 NZ_CP084741:1845480-1845716 [Pectobacterium carotovorum]
ATGACCGCGAAACCACGCAACACGCAGAGCGTGACCATAACGGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATTAATCTGAGTGCTACTCTGTCAACCGCATTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References