Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4079001..4079620 Replicon chromosome
Accession NZ_CP063911
Organism Klebsiella variicola strain M186-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA80_RS19730 Protein ID WP_002892050.1
Coordinates 4079001..4079219 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA80_RS19735 Protein ID WP_008805436.1
Coordinates 4079246..4079620 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA80_RS19695 4075044..4075307 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA80_RS19700 4075307..4075447 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA80_RS19705 4075444..4076142 - 699 WP_012968815.1 GNAT family N-acetyltransferase -
ISA80_RS19710 4076243..4077700 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
ISA80_RS19715 4077669..4078139 - 471 WP_008805434.1 YlaC family protein -
ISA80_RS19720 4078176..4078304 + 129 Protein_3840 hypothetical protein -
ISA80_RS19725 4078276..4078842 - 567 WP_032438710.1 maltose O-acetyltransferase -
ISA80_RS19730 4079001..4079219 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA80_RS19735 4079246..4079620 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA80_RS19740 4080103..4083249 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA80_RS19745 4083272..4084465 - 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179410 WP_002892050.1 NZ_CP063911:c4079219-4079001 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179410 NZ_CP084741:1035138-1035626 [Pectobacterium carotovorum]
GTGATCTCTGCTCCTGAGCCACTCCGCGCCGAACATATACTTTCCTCATTCTGTTGCGGCATGGAATCTATGGACAACTG
GCTGAAGCAACGGGCGATGAAAAATCAGGTCACTGGTGCATCACGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCCTCCAGTGCTGTAGCAACGAATGCCGCCCCCGGACGTTTTCGCCGCAATATGCCCGATCCGATC
CCGGTCGTGGTGCTGGGTCGTCTGGCGGTAGATAAATCATTACATGGTCAGGGTGTTGGCCGGGCGCTGGTACGAGATGC
TGGGATACGGGTGATTCAGGTGGCGGATACTATCGGCATCCGCGGGATGCTGGTTCATGCGCTGTCTGATGAGGCGCGTG
ATTTTTACCTGCGTGTGGGGTTTGAACCTTCACCGATTGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGCAGCG
ATTCACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179410 WP_008805436.1 NZ_CP063911:c4079620-4079246 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179410 NZ_CP084741:1034848-1035141 [Pectobacterium carotovorum]
ATGCCAGTGACGAATAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAACCCGCTGAACGCGATCTGATTGA
TCGTGCAGCGAAGGCCAGAGGAAAGAATCGCACTGATTTTGTCCTTGAGGCTGCGCGTGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAATTCCTTGCCCGTTTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGTAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References