Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2068218..2068993 Replicon chromosome
Accession NZ_CP063911
Organism Klebsiella variicola strain M186-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag ISA80_RS10105 Protein ID WP_032740995.1
Coordinates 2068218..2068703 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISA80_RS10110 Protein ID WP_004150912.1
Coordinates 2068700..2068993 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA80_RS10085 2065380..2066093 + 714 WP_008806478.1 DUF554 domain-containing protein -
ISA80_RS10095 2066608..2066824 - 217 Protein_1977 transposase -
ISA80_RS10100 2066921..2067514 - 594 WP_004188553.1 hypothetical protein -
ISA80_RS10105 2068218..2068703 - 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
ISA80_RS10110 2068700..2068993 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISA80_RS10115 2069589..2070965 + 1377 WP_162542795.1 cystathionine beta-synthase -
ISA80_RS10120 2070978..2072126 + 1149 WP_008806488.1 PLP-dependent aspartate aminotransferase family protein -
ISA80_RS10125 2072127..2073038 - 912 WP_023298220.1 LysR family transcriptional regulator -
ISA80_RS10130 2073136..2073738 + 603 WP_012540553.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2066672..2066824 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T179406 WP_032740995.1 NZ_CP063911:c2068703-2068218 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

>T179406 NZ_CP084741:c512110-511859 [Pectobacterium carotovorum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAGTATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCCAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179406 WP_004150912.1 NZ_CP063911:c2068993-2068700 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179406 NZ_CP084741:c511862-511608 [Pectobacterium carotovorum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGTATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGCATCACCGAAGAACACCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References