Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 912930..913520 Replicon chromosome
Accession NZ_CP063911
Organism Klebsiella variicola strain M186-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA80_RS04550 Protein ID WP_008804165.1
Coordinates 912930..913262 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA80_RS04555 Protein ID WP_012541132.1
Coordinates 913263..913520 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA80_RS04525 908968..909213 + 246 WP_008804171.1 signal transduction protein PmrD -
ISA80_RS04530 909344..910798 + 1455 WP_008804170.1 AMP nucleosidase -
ISA80_RS04540 911171..912607 - 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA80_RS04550 912930..913262 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA80_RS04555 913263..913520 - 258 WP_012541132.1 antitoxin Antitoxin
ISA80_RS04560 914049..915149 + 1101 WP_048268588.1 phosphotransferase -
ISA80_RS04565 915146..916117 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA80_RS04570 916323..917159 - 837 WP_008804155.1 alpha/beta hydrolase -
ISA80_RS04575 917336..917911 - 576 WP_077139353.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179404 WP_008804165.1 NZ_CP063911:c913262-912930 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179404 NZ_CP084741:c431188-430826 [Pectobacterium carotovorum]
ATGAGAGTCATCTCAGCCAAGGCCATCACCGATGCGATGAAAAAGCATGCGAAATGGAAAGTTGGTTTGAAGTTATGGCT
GGATGTTTTTGACAAGCCATCGCTGCGGTTTGAGTCCTACGAACAGTTATGTCATGTATGGCGAAATACATCTTGCTGGA
ATGTCGATCGCATACCTTTCGCATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGATATATATGTATTTGATATTAAT
GGTAACAATTGCAGAATCATAGCATGGTTAAACCCTAATACGGGTTCTTTGTATGTGAAAGACGTGTGCTCTCACGCAGA
GTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179404 WP_012541132.1 NZ_CP063911:c913520-913263 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179404 NZ_CP084741:c430822-430349 [Pectobacterium carotovorum]
ATGCGAAATACATCTCTGGGTTCTAACAGTTCTGTAGGAATCAGTGGGCTCATCGAAAATTTACCTCTTCTACTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTACTCTCTGCCTGTATGGAACCTATTGAAGACGATAATGATCTTCAGG
AAAGAATGGAGTGCATCAATCAACTTTACCTCTATTCCTCTGATGTCAATGATATTCCAGCCACTTTTGCAGAGCTCATC
GCGGACAGAGTCTATGAATATGAAAAGAAACACCGAGATGTGCCCCACGTCAGTCAGGCTGAAGCCTTAGCTTTTTTTAT
TGAAGAAAGAGGGCTGAAACAGTCAGATTTAAAGCAAATTGCAACGCAAAGCGTCATCTCTGACATTATTAACGGTAAGC
GAACCATGACATTACAGCAAGTTAAGGATTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References