Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1007904..1008494 Replicon chromosome
Accession NZ_CP063906
Organism Klebsiella pneumoniae strain BM327-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag ISA83_RS05170 Protein ID WP_022615588.1
Coordinates 1007904..1008236 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag ISA83_RS05175 Protein ID WP_000288812.1
Coordinates 1008237..1008494 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA83_RS05145 1003951..1004196 + 246 WP_004141189.1 signal transduction protein PmrD -
ISA83_RS05150 1004326..1005780 + 1455 WP_023301083.1 AMP nucleosidase -
ISA83_RS05160 1006146..1007582 - 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA83_RS05170 1007904..1008236 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA83_RS05175 1008237..1008494 - 258 WP_000288812.1 antitoxin Antitoxin
ISA83_RS05180 1008829..1009035 - 207 WP_032412591.1 helix-turn-helix domain-containing protein -
ISA83_RS05185 1009032..1009493 - 462 WP_032412594.1 hypothetical protein -
ISA83_RS05190 1009802..1010803 - 1002 WP_032412597.1 DUF4917 family protein -
ISA83_RS05195 1011075..1012310 + 1236 WP_021466313.1 DNA (cytosine-5-)-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T179347 WP_022615588.1 NZ_CP063906:c1008236-1007904 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T179347 NZ_CP084705:1476738-1477106 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT179347 WP_000288812.1 NZ_CP063906:c1008494-1008237 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179347 NZ_CP084705:1476520-1476741 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References