Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5466163..5466794 Replicon chromosome
Accession NZ_CP063898
Organism Klebsiella variicola strain BS326-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A1F2M5P8
Locus tag ISA87_RS26385 Protein ID WP_023323362.1
Coordinates 5466519..5466794 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag ISA87_RS26380 Protein ID WP_004181489.1
Coordinates 5466163..5466522 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA87_RS26355 5462420..5463364 + 945 WP_032733899.1 nickel ABC transporter permease subunit NikB -
ISA87_RS26360 5463361..5464194 + 834 WP_088717475.1 nickel ABC transporter permease subunit NikC -
ISA87_RS26365 5464194..5464958 + 765 WP_008806946.1 nickel import ATP-binding protein NikD -
ISA87_RS26370 5464955..5465746 + 792 WP_012540373.1 nickel import ATP-binding protein NikE -
ISA87_RS26375 5465734..5466135 + 402 WP_048268290.1 nickel-responsive transcriptional regulator NikR -
ISA87_RS26380 5466163..5466522 - 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISA87_RS26385 5466519..5466794 - 276 WP_023323362.1 type II toxin-antitoxin system HicA family toxin Toxin
ISA87_RS26390 5467191..5468204 + 1014 WP_023323363.1 magnesium transporter -
ISA87_RS26395 5468244..5469437 - 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
ISA87_RS26400 5469667..5471163 + 1497 WP_008806938.1 inorganic phosphate transporter PitA -
ISA87_RS26405 5471223..5471558 - 336 WP_002921203.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10260.86 Da        Isoelectric Point: 9.9665

>T179283 WP_023323362.1 NZ_CP063898:c5466794-5466519 [Klebsiella variicola]
MEQQVLSLRNKQHHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T179283 NZ_CP084656:2830710-2830964 [Pectobacterium versatile]
ATGAATAAACGACACCAAAGAACGCTATCAGATCTGTTTTCTCGTCCAGTTAATAGTTCAATAAAATGGAGCGATATAGA
ATCTCTTTTTATTGCTCTAGGGGCTGAAATTCATGAAAGGGAAGGTTCGAGGATTGCGGTATTGTTGAAAGGTGAGAAAA
AAATTTTTCATCGACCACATCCCAGACCGACTACTGACAAAGGAGCGGTTAACTCTATTCGGGTATGGTTAGAAAGTTTA
GGAGTAAGACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179283 WP_004181489.1 NZ_CP063898:c5466522-5466163 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179283 NZ_CP084656:2830961-2831302 [Pectobacterium versatile]
ATGATTAACACACTTCTTATCGACGGTCACACGGCCGTTATTGCTTTTGACTCGGAAATTGAAATGTTCCGGGGAGAATT
CATTGGCTTGAATGGCGGCGCTGATTTTTATGCTTACAGTGTTGATGAGCTGAAAAAAGAAGGGGCAACCTCTCTCGCTA
TTTTTCTTGATGAATGCCGCCGTGATGGTATTGAACCCTATAAATCCTACAGTGGAAAAGTCACAACACGTTTGACGCCA
GAACGTCATGCTGCGCTGGCGGTAACGGCGCAAGCAACTGGAGTTTCCATTAACGAATTGTTAAACGAAGGTGTCGATCT
GGTTCTTGAACGACGCTCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M5P8

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References