Detailed information of TA system TA179222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2879095..2879681
• Replicon: chromosome
• Accession: NZ_CP063889
• Organism: Klebsiella variicola strain BS325-3-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ISB13_RS13670
• Protein ID: WP_002920800.1
• Coordinates: 2879313..2879681 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: ISB13_RS13665
• Protein ID: WP_032731361.1
• Coordinates: 2879095..2879316 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB13_RS13645	2875252..2876178	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ISB13_RS13650	2876175..2877452	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ISB13_RS13655	2877449..2878216	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISB13_RS13660	2878218..2878931	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISB13_RS13665	2879095..2879316	+	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISB13_RS13670	2879313..2879681	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISB13_RS13675	2879973..2881289	+	1317	WP_194386930.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISB13_RS13680	2881396..2882283	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISB13_RS13685	2882280..2883125	+	846	WP_032740290.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISB13_RS13690	2883127..2884197	+	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2876175..2884934	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T179222 WP_002920800.1 NZ_CP063889:2879313-2879681 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179222 NZ_CP084650:c3003116-3002694 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT179222 WP_032731361.1 NZ_CP063889:2879095-2879316 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179222 NZ_CP084650:c3003363-3003097 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A1F2M0C2