Detailed information of TA system TA179218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1156613..1157210
• Replicon: chromosome
• Accession: NZ_CP063889
• Organism: Klebsiella variicola strain BS325-3-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISB13_RS05660
• Protein ID: WP_012542526.1
• Coordinates: 1156893..1157210 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISB13_RS05655
• Protein ID: WP_012542525.1
• Coordinates: 1156613..1156900 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB13_RS05625	1152473..1152721	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
ISB13_RS05630	1152738..1153079	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISB13_RS05635	1153110..1154225	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISB13_RS05640	1154405..1154986	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISB13_RS05645	1154986..1155354	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISB13_RS05650	1155474..1156127	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISB13_RS05655	1156613..1156900	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISB13_RS05660	1156893..1157210	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISB13_RS05665	1157395..1158438	-	1044	WP_023297181.1	hypothetical protein	-
ISB13_RS05670	1158968..1159834	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISB13_RS05675	1159943..1161370	+	1428	WP_194387267.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179218 WP_012542526.1 NZ_CP063889:c1157210-1156893 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179218 NZ_CP084650:c974758-974555 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATACGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179218 WP_012542525.1 NZ_CP063889:c1156900-1156613 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179218 NZ_CP084650:c975178-974810 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2