Detailed information of TA system TA179186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1533024..1533670
• Replicon: chromosome
• Accession: NZ_CP063885
• Organism: Klebsiella pneumoniae strain BS361-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ISB15_RS07505
• Protein ID: WP_194429468.1
• Coordinates: 1533323..1533670 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISB15_RS07500
• Protein ID: WP_194429469.1
• Coordinates: 1533024..1533323 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB15_RS07480	1529230..1529988	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
ISB15_RS07485	1530038..1530868	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
ISB15_RS07490	1530919..1531248	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
ISB15_RS07495	1531453..1532961	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
ISB15_RS07500	1533024..1533323	-	300	WP_194429469.1	helix-turn-helix transcriptional regulator	Antitoxin
ISB15_RS07505	1533323..1533670	-	348	WP_194429468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISB15_RS07510	1533846..1536293	-	2448	WP_002920561.1	glycogen phosphorylase	-
ISB15_RS07515	1536311..1537744	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13419.41 Da
• Isoelectric Point: 6.2327

>T179186 WP_194429468.1 NZ_CP063885:c1533670-1533323 [Klebsiella pneumoniae]
MWDVETTDTFDTGFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T179186 NZ_CP084642:2669093-2669296 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10813.63 Da
• Isoelectric Point: 10.6143

>AT179186 WP_194429469.1 NZ_CP063885:c1533323-1533024 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAERRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNAPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT179186 NZ_CP084642:2668673-2669041 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA