Detailed information of TA system TA179137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 70852..71579
• Replicon: plasmid pS174-1.3
• Accession: NZ_CP063877
• Organism: Klebsiella quasipneumoniae strain S174-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IOD26_RS27435
• Protein ID: WP_011251285.1
• Coordinates: 71268..71579 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD26_RS27430
• Protein ID: WP_011251286.1
• Coordinates: 70852..71271 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD26_RS27400	66295..67050	+	756	WP_017901462.1	hypothetical protein	-
IOD26_RS27405	67243..67767	+	525	WP_017901461.1	hypothetical protein	-
IOD26_RS27410	67924..68891	+	968	Protein_72	IS5 family transposase	-
IOD26_RS27415	68985..69251	-	267	WP_017900868.1	hypothetical protein	-
IOD26_RS27420	69355..70323	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
IOD26_RS27425	70369..70782	-	414	WP_017901337.1	hypothetical protein	-
IOD26_RS27430	70852..71271	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IOD26_RS27435	71268..71579	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IOD26_RS27440	71784..72242	-	459	Protein_78	DDE-type integrase/transposase/recombinase	-
IOD26_RS27445	72335..72712	+	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
IOD26_RS27450	72709..73056	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
IOD26_RS27455	73105..74643	+	1539	WP_017901237.1	IS66 family transposase	-
IOD26_RS27460	74683..74862	+	180	Protein_82	IS5/IS1182 family transposase	-
IOD26_RS27465	75408..76388	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	1..159310	159310
	-	inside	IScluster/Tn	-	-	56268..88242	31974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T179137 WP_011251285.1 NZ_CP063877:c71579-71268 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T179137 NZ_CP084588:c63618-63325 [Mycobacterium intracellulare subsp. chimaera]
GTGAGCGCATACGTCCTTTCACCCGCTGCGCAAGCAGATTTGGAACAGATCTGGGACTACACGCACGAGCACTGGGGCCT
GGATCAGGCTGAGCAGTACCTGCGAGAACTTCAACGCGCTATCGAGCGCGCAGCGGCGAACCCCCGGATCGGGCGGGCCT
GCGACGAGATCCGCCCGGGCTATCGCAAGCTGGCAGCCGGGTCGCACATGCTGTTTTATCGGGTGACAACCCAAGGCTTC
CTCGATGTCGTGCGTGTCCTGCACCAACGCATGGACGTCGACCGCCACCTTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT179137 WP_011251286.1 NZ_CP063877:c71271-70852 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT179137 NZ_CP084588:c63866-63615 [Mycobacterium intracellulare subsp. chimaera]
GTGGGTAAAAACACGTCCTTCAGTCTCGATGAGCATTACAGCGCCTTCATTGAGGCTGAGGTGGCTGCGGGACGTTACCG
GTCGGCCAGTGATGTCGTGCGCTCCGCGTTGCGGTTACTCGAGGACCGTGAAATTCAGCTTCGGGCACTGCGCGAGGCGC
TAGTGGCCGGTGAGCGCAGCGGCACTTCAACACCGTTTGACTTCGACGCCTTCCTCGACGGCAAACGCGCCGAAAAGCCG
CAAGGCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1