Detailed information of TA system TA179093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 158833..159355
• Replicon: plasmid pM142-3
• Accession: NZ_CP063868
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: ISD32_RS27850
• Protein ID: WP_044265921.1
• Coordinates: 158833..159117 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: ISD32_RS27855
• Protein ID: WP_004187025.1
• Coordinates: 159107..159355 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS27830	154172..155101	-	930	WP_094354314.1	LysR family transcriptional regulator	-
ISD32_RS27835	155201..157054	+	1854	WP_094354313.1	dihydroxy-acid dehydratase	-
ISD32_RS27840	157137..157459	+	323	Protein_173	Tn3 family transposase	-
ISD32_RS27845	157543..158817	+	1275	WP_044265918.1	ISNCY family transposase	-
ISD32_RS27850	158833..159117	-	285	WP_044265921.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISD32_RS27855	159107..159355	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
ISD32_RS27860	159764..160231	-	468	WP_044265924.1	GNAT family N-acetyltransferase	-
ISD32_RS27865	160617..161174	+	558	WP_044265927.1	OsmC family protein	-
ISD32_RS27870	161361..161930	+	570	WP_044265930.1	TetR/AcrR family transcriptional regulator	-
ISD32_RS27875	161948..162286	+	339	WP_044265932.1	carboxymuconolactone decarboxylase family protein	-
ISD32_RS27880	162695..163063	+	369	Protein_181	DUF4102 domain-containing protein	-
ISD32_RS27885	163066..163278	+	213	Protein_182	DUF3363 domain-containing protein	-
ISD32_RS27890	163307..164128	-	822	WP_044265935.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..180701	180701
	-	flank	IS/Tn	-	-	157165..157263	98

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11012.73 Da
• Isoelectric Point: 10.5388

>T179093 WP_044265921.1 NZ_CP063868:c159117-158833 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTLQVHFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T179093 NZ_CP084532:c989037-988510 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT179093 WP_004187025.1 NZ_CP063868:c159355-159107 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT179093 NZ_CP084532:c989324-989034 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4