Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4737889..4738546 Replicon chromosome
Accession NZ_CP063867
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISD32_RS22815 Protein ID WP_002916310.1
Coordinates 4738136..4738546 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISD32_RS22810 Protein ID WP_002916312.1
Coordinates 4737889..4738155 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS22790 4734332..4735060 - 729 WP_101859396.1 MurR/RpiR family transcriptional regulator -
ISD32_RS22795 4735111..4735422 + 312 WP_101859397.1 N(4)-acetylcytidine aminohydrolase -
ISD32_RS22800 4735586..4736245 + 660 WP_008806429.1 hemolysin III family protein -
ISD32_RS22805 4736660..4737643 - 984 WP_087498967.1 tRNA-modifying protein YgfZ -
ISD32_RS22810 4737889..4738155 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISD32_RS22815 4738136..4738546 + 411 WP_002916310.1 protein YgfX Toxin
ISD32_RS22820 4738553..4739074 - 522 WP_008806427.1 flavodoxin FldB -
ISD32_RS22825 4739175..4740071 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
ISD32_RS22830 4740094..4740807 + 714 WP_008806425.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISD32_RS22835 4740813..4742546 + 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179089 WP_002916310.1 NZ_CP063867:4738136-4738546 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179089 NZ_CP084532:310524-310892 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179089 WP_002916312.1 NZ_CP063867:4737889-4738155 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179089 NZ_CP084532:310306-310527 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References