Detailed information of TA system TA179082

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2781775..2782467
• Replicon: chromosome
• Accession: NZ_CP063867
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ISD32_RS13475
• Protein ID: WP_110224310.1
• Coordinates: 2782129..2782467 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: ISD32_RS13470
• Protein ID: WP_074186702.1
• Coordinates: 2781775..2782104 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS13440	2777428..2777940	-	513	WP_110224303.1	hypothetical protein	-
ISD32_RS13445	2778206..2778421	-	216	WP_048291277.1	AlpA family phage regulatory protein	-
ISD32_RS13450	2778539..2779204	-	666	WP_110224305.1	inovirus Gp2 family protein	-
ISD32_RS13455	2779830..2780780	+	951	WP_110224307.1	hypothetical protein	-
ISD32_RS13460	2781009..2781251	+	243	WP_048291516.1	hypothetical protein	-
ISD32_RS13465	2781272..2781751	+	480	WP_048291275.1	DNA repair protein RadC	-
ISD32_RS13470	2781775..2782104	+	330	WP_074186702.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ISD32_RS13475	2782129..2782467	+	339	WP_110224310.1	TA system toxin CbtA family protein	Toxin
ISD32_RS13480	2782859..2783608	+	750	WP_048291272.1	hypothetical protein	-
ISD32_RS13485	2784702..2785445	-	744	WP_008805296.1	ABC transporter permease	-
ISD32_RS13490	2785442..2786632	-	1191	WP_008805295.1	ABC transporter substrate-binding protein	-
ISD32_RS13495	2786616..2787416	-	801	WP_008805294.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2770589..2790684	20095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (9-108)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12897.89 Da
• Isoelectric Point: 9.1652

>T179082 WP_110224310.1 NZ_CP063867:2782129-2782467 [Klebsiella variicola]
MKTLPANQRVAKPCPPPVLVWQMLLTRLLEQHYGLTLNDTPFSDENVIQEHINAGITLADAINFLVEKYELVRIDRRGFN
CQEQSPYLRAVDILRARQATGLLRQRHHPSAR

Nucleotide

• Length: 339 bp

>T179082 NZ_CP084529:c4095127-4094753 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12373.85 Da
• Isoelectric Point: 5.8867

>AT179082 WP_074186702.1 NZ_CP063867:2781775-2782104 [Klebsiella variicola]
MDKETDTVRWGLNRPGSPQLGARLVQEGNRLHYLADRAGTTGAFNEEECHQLNSAFPHFIEQMELMLLSGELNPRHAHRV
TLYHNELTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 330 bp

>AT179082 NZ_CP084529:c4095584-4095216 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA