Detailed information of TA system TA179035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1752509..1753248
• Replicon: chromosome
• Accession: NZ_CP063863
• Organism: Klebsiella pneumoniae strain M63-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IOD47_RS08235
• Protein ID: WP_032415800.1
• Coordinates: 1752763..1753248 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IOD47_RS08230
• Protein ID: WP_003026799.1
• Coordinates: 1752509..1752775 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD47_RS08215	1748012..1750081	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
IOD47_RS08220	1750378..1751747	+	1370	WP_087830479.1	IS3 family transposase	-
IOD47_RS08225	1751948..1752376	+	429	WP_004901287.1	GFA family protein	-
IOD47_RS08230	1752509..1752775	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IOD47_RS08235	1752763..1753248	+	486	WP_032415800.1	GNAT family N-acetyltransferase	Toxin
IOD47_RS08240	1753592..1753744	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
IOD47_RS08245	1754046..1755665	+	1620	WP_032415799.1	ATP-binding cassette domain-containing protein	-
IOD47_RS08250	1755764..1755976	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
IOD47_RS08255	1756229..1756519	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
IOD47_RS08260	1756765..1758120	-	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1751115..1751747	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17624.46 Da
• Isoelectric Point: 10.1428

>T179035 WP_032415800.1 NZ_CP063863:1752763-1753248 [Klebsiella pneumoniae]
VGRITAPEPLCSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T179035 NZ_CP084504:46574-47056 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179035 WP_003026799.1 NZ_CP063863:1752509-1752775 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179035 NZ_CP084504:46320-46586 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66