Detailed information of TA system TA179022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67756..68492
• Replicon: plasmid pM1026-3Ar.1
• Accession: NZ_CP063859
• Organism: Klebsiella pneumoniae strain M1026-3Ar

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: IOD46_RS25300
• Protein ID: WP_004187044.1
• Coordinates: 68010..68492 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IOD46_RS25295
• Protein ID: WP_003026799.1
• Coordinates: 67756..68022 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD46_RS25280	63228..64622	+	1395	WP_032439652.1	cytosine permease	-
IOD46_RS25285	64634..65842	+	1209	WP_032448288.1	imidazolonepropionase	-
IOD46_RS25290	65835..66644	+	810	WP_023329017.1	N-formylglutamate deformylase	-
IOD46_RS25295	67756..68022	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IOD46_RS25300	68010..68492	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
IOD46_RS25305	68703..70049	+	1347	WP_077251107.1	ISNCY family transposase	-
IOD46_RS25310	70209..70913	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
IOD46_RS25315	71190..72536	+	1347	WP_077254728.1	ISNCY family transposase	-
IOD46_RS25320	72585..72983	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
IOD46_RS25325	73131..73238	-	108	Protein_76	IS3 family transposase	-
IOD46_RS25330	73236..73406	-	171	Protein_77	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / catA2 / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B)	iutA / iucD / iucC / iucB / iucA	1..262519	262519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T179022 WP_004187044.1 NZ_CP063859:68010-68492 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T179022 NZ_CP084502:345051-345419 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTATTAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179022 WP_003026799.1 NZ_CP063859:67756-68022 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179022 NZ_CP084502:344833-345054 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66