Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2560269..2560894 Replicon chromosome
Accession NZ_CP063851
Organism Klebsiella pneumoniae strain M1023-4Ar

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag IOD43_RS12210 Protein ID WP_019705794.1
Coordinates 2560269..2560652 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD43_RS12215 Protein ID WP_004150355.1
Coordinates 2560652..2560894 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD43_RS12195 2557635..2558537 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IOD43_RS12200 2558534..2559169 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD43_RS12205 2559166..2560095 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD43_RS12210 2560269..2560652 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD43_RS12215 2560652..2560894 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD43_RS12220 2561099..2562016 + 918 WP_004146235.1 alpha/beta hydrolase -
IOD43_RS12225 2562031..2562972 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IOD43_RS12230 2563017..2563454 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD43_RS12235 2563451..2564311 - 861 WP_004146232.1 virulence factor BrkB family protein -
IOD43_RS12240 2564305..2564904 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T178966 WP_019705794.1 NZ_CP063851:c2560652-2560269 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T178966 NZ_CP084443:c1655874-1655704 [Staphylococcus lugdunensis]
ATGTCAAAAGAAAAAAAGAGTCGTTTAAATTATCATGAGGAAGAGAATGCGATGGTTGAAAATTTAGAAGACTTAAAAGA
GCTGGGCAAAGAAATGGAACACATCTCAGAGAAAAATGACGAAGAAAAAGTTAATCAATCGCATGATACATCAAAGTCTT
CAAATTCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT178966 WP_004150355.1 NZ_CP063851:c2560894-2560652 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT178966 NZ_CP084443:c1656513-1655914 [Staphylococcus lugdunensis]
ATGGCAATGAACTTTAAAGTATTTGAAAATAAAGATCAAGTTGCAGAGTATTCAGCAGATATCATTAGAAAACAATTTAA
CAATAATCCTACTACAATTGCTGGATTTCATTTAAACAAAGAGGCATCACCAGTACTTGATATGCTTAAAAAGAATGTTG
ACCAACATGCGGTTGACTTCAGCCAAATTAACATTCTTGACTATGATCAAAATCAAGAATATTTCAAAGCGCTGGGGGTT
CCTGAGAGTCAAATTTATCCTATCTCATACGATCAAGATGCTGAAGCGTTTATCTCTGATAAAATCAAAACAAAAGATAA
TAAAGGGAAACTAATATTACAAGTAGCTTCAATTGATGATCAAGGAAACCTTAATATCAGTCTACGTCAAGGATTGTTAA
ATGCTAGAGAAATTATCTTAGTCATTACAGGACATAATAAAAAAGAAGTAGTAGAAAAACTGTATAATGAAAATGGAAAA
TCTAATTTTGAACCTGCTGATTTAAAAGCACATCGTATGGTAACGGTTATTTTAGACGAAGCGGCAGCTGAAGGTTTGCC
AGAAGATGTACGTCATTACTTTACTGATCGGTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References