Detailed information of TA system TA178805

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3405000..3405835
• Replicon: chromosome
• Accession: NZ_CP063774
• Organism: Escherichia coli O25b:H4-ST131

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: INT78_RS16170
• Protein ID: WP_000854759.1
• Coordinates: 3405458..3405835 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: INT78_RS16165
• Protein ID: WP_001295723.1
• Coordinates: 3405000..3405368 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT78_RS16135	3400014..3400760	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
INT78_RS16140	3400775..3402316	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
INT78_RS16145	3402509..3403246	+	738	Protein_3148	DUF945 domain-containing protein	-
INT78_RS16150	3403588..3404061	+	474	WP_001350782.1	antirestriction protein	-
INT78_RS16155	3404077..3404553	+	477	WP_001186775.1	RadC family protein	-
INT78_RS16160	3404616..3404837	+	222	WP_000692345.1	DUF987 domain-containing protein	-
INT78_RS16165	3405000..3405368	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
INT78_RS16170	3405458..3405835	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
INT78_RS16175	3405832..3406320	+	489	WP_000761690.1	hypothetical protein	-
INT78_RS16180	3406337..3406513	+	177	WP_000839286.1	DUF957 domain-containing protein	-
INT78_RS16185	3406613..3406771	+	159	WP_001467148.1	hypothetical protein	-
INT78_RS16190	3407135..3407311	+	177	Protein_3157	helix-turn-helix domain-containing protein	-
INT78_RS16195	3408084..3409724	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T178805 WP_000854759.1 NZ_CP063774:3405458-3405835 [Escherichia coli O25b:H4-ST131]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T178805 NZ_CP084301:c22126-21830 [Acinetobacter pseudolwoffii]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATTCGCCTGAA
TCGTAGATTAGATAAGGTTCAACGTGGAAATTGGGGAGACATTAAGCCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTGCTGGATGGAGAATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGAGGAGATAAATCG
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT178805 WP_001295723.1 NZ_CP063774:3405000-3405368 [Escherichia coli O25b:H4-ST131]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT178805 NZ_CP084301:c21828-21538 [Acinetobacter pseudolwoffii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TTGCTAAAGAAGCAGGGATTGGGCGTGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACCATT
AATCGTGTAGTGAATGCTTTAGGCTTAAAACTCACAGTACAACATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52