Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 65021..65622 Replicon plasmid pEH10-18-47_3
Accession NZ_CP063502
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag IQS20_RS22795 Protein ID WP_001216034.1
Coordinates 65021..65401 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IQS20_RS22800 Protein ID WP_001190712.1
Coordinates 65401..65622 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS22770 60462..61946 - 1485 WP_000124159.1 hypothetical protein -
IQS20_RS22775 61946..63139 - 1194 WP_032214401.1 hypothetical protein -
IQS20_RS22780 63225..63677 - 453 WP_001326849.1 late promoter-activating protein -
IQS20_RS22785 63766..64809 - 1044 WP_112918711.1 DUF968 domain-containing protein -
IQS20_RS22790 64837..65016 - 180 WP_000113018.1 hypothetical protein -
IQS20_RS22795 65021..65401 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IQS20_RS22800 65401..65622 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IQS20_RS22805 65695..66084 - 390 WP_000506724.1 DNA repair protein -
IQS20_RS22810 66259..66591 + 333 Protein_77 DNA-binding protein -
IQS20_RS22815 66625..66843 + 219 WP_094318256.1 hypothetical protein -
IQS20_RS22820 66844..67200 + 357 WP_001062545.1 hypothetical protein -
IQS20_RS22825 68276..68458 - 183 WP_001548895.1 hypothetical protein -
IQS20_RS22830 68634..69107 - 474 WP_077632598.1 hypothetical protein -
IQS20_RS22835 69264..69812 - 549 Protein_82 hypothetical protein -
IQS20_RS22840 70007..70513 - 507 WP_000021754.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A) / floR / qnrS1 - 1..89721 89721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T178535 WP_001216034.1 NZ_CP063502:c65401-65021 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T178535 NZ_CP084096:c3014352-3013030 [Escherichia coli O78]
ATGCCCAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACTTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCTCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGAATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGGTATG
ATTTTATGAAATTCCAGGTCTTTCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGTACTGGATAACGTGAAGACTTCATTACCAACAGATTTTCCAGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT178535 WP_001190712.1 NZ_CP063502:c65622-65401 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT178535 NZ_CP084096:c3014618-3014352 [Escherichia coli O78]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAAAAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References