Detailed information of TA system TA178523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1286991..1287517
• Replicon: chromosome
• Accession: NZ_CP063499
• Organism: Escherichia coli strain EH10-18-47

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IQS20_RS06120
• Protein ID: WP_000323025.1
• Coordinates: 1286991..1287278 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IQS20_RS06125
• Protein ID: WP_000534858.1
• Coordinates: 1287278..1287517 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS20_RS06090	1282050..1282478	-	429	WP_000506936.1	cell envelope integrity protein TolA	-
IQS20_RS06095	1283522..1284064	-	543	WP_040062257.1	DUF1133 family protein	-
IQS20_RS06100	1284061..1284351	-	291	WP_040062258.1	DUF1364 domain-containing protein	-
IQS20_RS06105	1284351..1284950	-	600	WP_040062259.1	DUF1367 family protein	-
IQS20_RS06110	1285458..1286570	+	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
IQS20_RS06115	1286764..1286919	-	156	WP_096282653.1	type I toxin-antitoxin system toxin HokD	-
IQS20_RS06120	1286991..1287278	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IQS20_RS06125	1287278..1287517	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IQS20_RS06130	1287542..1287847	+	306	WP_071593935.1	hypothetical protein	-
IQS20_RS06135	1288050..1288382	+	333	WP_001301033.1	protein FlxA	-
IQS20_RS06140	1288594..1289291	-	698	Protein_1204	IS1 family transposase	-
IQS20_RS06145	1289349..1290194	+	846	Protein_1205	site-specific integrase	-
IQS20_RS06150	1290246..1291181	+	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1223448..1302721	79273
	-	inside	IScluster/Tn	-	-	1285458..1289291	3833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T178523 WP_000323025.1 NZ_CP063499:c1287278-1286991 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T178523 NZ_CP084096:c428385-427900 [Escherichia coli O78]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCGCGTGTTTACGTTATCTGTATTGCCAATACTCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCTTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT178523 WP_000534858.1 NZ_CP063499:c1287517-1287278 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT178523 NZ_CP084096:c428654-428376 [Escherichia coli O78]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGGGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCGTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8