Detailed information of TA system TA178436

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20192..20618
• Replicon: plasmid pEM03-18-08_1
• Accession: NZ_CP063484
• Organism: Escherichia coli strain EM03-18-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IQS21_RS22985
• Protein ID: WP_001312861.1
• Coordinates: 20460..20618 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20192..20416 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS21_RS22950	15383..15643	+	261	WP_071600428.1	hypothetical protein	-
IQS21_RS22955	16116..16655	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
IQS21_RS22960	16718..16951	+	234	WP_000005990.1	DUF905 family protein	-
IQS21_RS22965	17017..18975	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
IQS21_RS22970	19030..19464	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
IQS21_RS22975	19461..20180	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
IQS21_RS22980	20192..20380	-	189	WP_001299721.1	hypothetical protein	-
-	20192..20416	+	225	NuclAT_0	-	Antitoxin
-	20192..20416	+	225	NuclAT_0	-	Antitoxin
-	20192..20416	+	225	NuclAT_0	-	Antitoxin
-	20192..20416	+	225	NuclAT_0	-	Antitoxin
IQS21_RS22985	20460..20618	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS21_RS22990	21540..21827	+	288	WP_065800506.1	hypothetical protein	-
IQS21_RS22995	21945..22766	+	822	WP_001234469.1	DUF945 domain-containing protein	-
IQS21_RS23000	23063..23653	-	591	WP_165899088.1	transglycosylase SLT domain-containing protein	-
IQS21_RS23005	23986..24369	+	384	WP_001151524.1	relaxosome protein TraM	-
IQS21_RS23010	24556..25185	+	630	WP_152241308.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-3.5	-	1..77443	77443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178436 WP_001312861.1 NZ_CP063484:20460-20618 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178436 NZ_CP084023:4266087-4266341 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAAAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAATCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCAGCCTGC
CGCTACCACTATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT178436 NZ_CP063484:20192-20416 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin