Detailed information of TA system TA178330

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 318409..319244
• Replicon: chromosome
• Accession: NZ_CP063473
• Organism: Escherichia coli strain EM18-18-42

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: IQS24_RS01440
• Protein ID: WP_001094445.1
• Coordinates: 318409..318786 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IQS24_RS01445
• Protein ID: WP_024174331.1
• Coordinates: 318876..319244 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS24_RS01420	314501..316039	-	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-
IQS24_RS01425	316788..317630	-	843	WP_001280501.1	DUF4942 domain-containing protein	-
IQS24_RS01430	317715..317912	-	198	WP_000838536.1	DUF957 domain-containing protein	-
IQS24_RS01435	317924..318412	-	489	WP_205595556.1	hypothetical protein	-
IQS24_RS01440	318409..318786	-	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
IQS24_RS01445	318876..319244	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQS24_RS01450	319318..319539	-	222	WP_000692346.1	DUF987 domain-containing protein	-
IQS24_RS01455	319608..320084	-	477	WP_001186725.1	RadC family protein	-
IQS24_RS01460	320100..320585	-	486	WP_205595557.1	antirestriction protein	-
IQS24_RS01465	320677..321495	-	819	WP_001234749.1	DUF945 domain-containing protein	-
IQS24_RS01470	321586..321819	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	307013..341707	34694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T178330 WP_001094445.1 NZ_CP063473:c318786-318409 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T178330 NZ_CP083947:415483-415869 [Acinetobacter johnsonii]
ATGGAACATCGTCATTTTCAACTTAAACCCAGCCGCCTGGCTTATGTGTTTCAGTTGTCGATTTATGCCGTTCTGATGCT
GGTCTTGTATCAGCTACTTCCTTTAGGAGTGTGGCTGATCGCTTTGGTTGCAGGCTTTGTGCTGTATTTGTTTTTTCTAC
GTGGGCCGCAACATTTTCAGCTTCAGCATTTAGATGGTCGTGATTGGTCTTTGTCACTTCAGAAACAAACCAATGTGCAA
CGAGTGCAAATCAGCCATATTGTGGATCATCGTGCTTATATTGTGATTTATTTTCAACACTTTAGAGCAAAACCGATGTT
GGTTTGGTGTGATCAGTTACCCATCACACAATGGAAAGCTCTGAAAATACTCACTAAAATGCAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT178330 WP_024174331.1 NZ_CP063473:c319244-318876 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT178330 NZ_CP083947:415220-415477 [Acinetobacter johnsonii]
ATGACTGAAGAAATGAGCCTAGAAGAGCGTAAAGTAATTTATCGTGCACGTCGTGGTTTAAAAGAAATTGATGTGTACTT
TGACCCTTATGTGAAAAATTATTATCTTCAGGCGGATCCTGCGGAAAAGGCCATGTTTGCGGCCTTGGTTGAACAAGAAG
ATCCAGATTTATTGGATTGGTTTATGGAAGTCTCTGAGCCGCCACGCCCTGAAATGAAAACCTTGATTCAAAAATTAAAG
CATTATGTACACGGATAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6