Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2963706..2964208 Replicon chromosome
Accession NZ_CP063459
Organism Escherichia coli strain EM22-18-53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS26_RS14155 Protein ID WP_000767829.1
Coordinates 2963954..2964208 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS26_RS14150 Protein ID WP_001259255.1
Coordinates 2963706..2963957 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS26_RS14125 2958885..2959952 - 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IQS26_RS14130 2959952..2961022 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
IQS26_RS14135 2961019..2962323 - 1305 WP_075826842.1 histidinol dehydrogenase -
IQS26_RS14140 2962329..2963228 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
IQS26_RS14145 2963374..2963424 - 51 WP_001364200.1 his operon leader peptide -
IQS26_RS14150 2963706..2963957 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS26_RS14155 2963954..2964208 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS26_RS14160 2964291..2965115 + 825 WP_075826841.1 SDR family oxidoreductase -
IQS26_RS14165 2965161..2966090 + 930 WP_000803351.1 LysR family transcriptional regulator -
IQS26_RS14170 2966305..2966367 + 63 WP_010723108.1 membrane protein YoeI -
IQS26_RS14175 2966357..2967715 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS26_RS14180 2967894..2968952 + 1059 WP_000492346.1 transport protein YeeE -
IQS26_RS14185 2968966..2969193 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178283 WP_000767829.1 NZ_CP063459:2963954-2964208 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178283 NZ_CP083911:1711566-1711979 [Tepidimonas taiwanensis]
TTGAGCTACCTCATCGATACCAACGTCCTGTCCGAACTGCGGCGCAGGTCTCCGGACCCCGGTGTGGCCGCGTGGTTTGC
GCAGCGGCCCGCCACGACGCTGTACGTATCGGTCCTGACCCTCGGCGAGATTCGCAAGGGCATCGAGGGGGTGACGGACG
ACCGCCGGCGACAGGCCCTGCTCGATTGGCTGGAAACGGATTTGCCGGCTTTTTTCACCGGGCGCGTGCTGCCGATCGAC
CGCGCCGTCGCCGATCGTTGGGGCCGGCTGGTGGCCGCGGCAGGCCGCCCACTGCCCGCGATCGAAAGCCTGCTGGCCGC
CACGGCGCTGACCCACGACCTGGTGCTGGTCACACGCAACAGCAAGGATTTCGCCGGTCTGCCCGTCGTGACCCTCAATC
CCTGGTCCACCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178283 WP_001259255.1 NZ_CP063459:2963706-2963957 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178283 NZ_CP083911:1711321-1711569 [Tepidimonas taiwanensis]
ATGCAGACATGGCAAATGCAACAAGCCAAAGCCCGGTTGTCCGAGCTGGTCAAGTGCGCCCGCGAACAGCCCCAGGCGAT
CACGGTCCACGGTGAACCGGTGGCCATCGTCGTCTCGCGCGAGACGTTCGAACGTCTGGCCCAGGCGAGCGAGTCGCTGC
TGGACTTCATGCGGCGCTCGCCGCTCTACGGCGAGGAGGACATCGCTTTCGCGCGTGATCCGAGCCGCACGCGCGAGGTG
GTGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References