Detailed information of TA system TA178145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5602806..5603401
• Replicon: chromosome
• Accession: NZ_CP063396
• Organism: Pseudomonas aeruginosa strain ST167_d26burn

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IPV38_RS26000
• Protein ID: WP_003113526.1
• Coordinates: 5603123..5603401 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IPV38_RS25995
• Protein ID: WP_003113527.1
• Coordinates: 5602806..5603111 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV38_RS25970 (IPV38_25965)	5597964..5598299	+	336	WP_034035738.1	hypothetical protein	-
IPV38_RS25975 (IPV38_25970)	5598309..5598698	+	390	WP_096219661.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
IPV38_RS25980 (IPV38_25975)	5598691..5599515	+	825	WP_096219662.1	HNH endonuclease	-
IPV38_RS25985 (IPV38_25980)	5599891..5601651	-	1761	WP_132764182.1	HEPN domain-containing protein	-
IPV38_RS25990 (IPV38_25985)	5602056..5602469	-	414	WP_049876905.1	hypothetical protein	-
IPV38_RS25995 (IPV38_25990)	5602806..5603111	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IPV38_RS26000 (IPV38_25995)	5603123..5603401	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IPV38_RS26005	5603454..5603582	-	129	Protein_5148	integrase	-
IPV38_RS26010 (IPV38_26000)	5603730..5605958	+	2229	WP_023086667.1	TonB-dependent receptor	-
IPV38_RS26015 (IPV38_26005)	5606028..5606675	-	648	WP_003095021.1	carbonate dehydratase	-
IPV38_RS26020 (IPV38_26010)	5606737..5607975	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T178145 WP_003113526.1 NZ_CP063396:c5603401-5603123 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T178145 NZ_CP083869:998522-998857 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT178145 WP_003113527.1 NZ_CP063396:c5603111-5602806 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT178145 NZ_CP083869:998274-998522 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3