Detailed information of TA system TA177748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 25229..26139
• Replicon: plasmid pHKU57_1
• Accession: NZ_CP063216
• Organism: Klebsiella pneumoniae strain HKU57

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IM695_RS25295
• Protein ID: WP_032413375.1
• Coordinates: 25669..26139 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: IM695_RS25290
• Protein ID: WP_032413443.1
• Coordinates: 25229..25672 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM695_RS25260	20913..21584	-	672	WP_159226239.1	alpha/beta hydrolase	-
IM695_RS25265	21675..22643	-	969	WP_077268726.1	IS5-like element IS903B family transposase	-
IM695_RS25270	23059..23607	+	549	WP_023320124.1	GNAT family N-acetyltransferase	-
IM695_RS25275	23730..24311	-	582	WP_077254766.1	transposase	-
IM695_RS25280	24331..24516	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
IM695_RS25285	24617..25129	+	513	Protein_29	DUF4113 domain-containing protein	-
IM695_RS25290	25229..25672	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
IM695_RS25295	25669..26139	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
IM695_RS25300	26249..26509	-	261	WP_017901409.1	hypothetical protein	-
IM695_RS25305	27081..27458	-	378	Protein_33	transposase	-
IM695_RS25310	27532..27725	+	194	Protein_34	transposase	-
IM695_RS25315	27848..28798	+	951	WP_077268727.1	IS5-like element IS903B family transposase	-
IM695_RS25320	28851..29276	+	426	Protein_36	hypothetical protein	-
IM695_RS25325	29277..29432	-	156	Protein_37	transposase	-
IM695_RS25330	30168..30260	-	93	Protein_38	hypothetical protein	-
IM695_RS25335	30302..30838	+	537	WP_032437437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / dfrA25 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / blaDHA-1 / qnrB4	-	1..273789	273789
	-	inside	IScluster/Tn	-	-	17484..34383	16899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T177748 WP_032413375.1 NZ_CP063216:25669-26139 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T177748 NZ_CP083648:c1863381-1863100 [Corynebacterium coyleae]
ATGATCCAGTCATTCGCTGACAAGGACACTGAGCGTCTGTGGAATCGAGAGCGGGTCCGCTCGATCGATTCCCGCATTCA
CTCAGTAGCGCTGCGCAAGCTTCGCCAGCTTGGGTACGCGCAGACTCTCGATGAGTTACGGATTCCTCCGGGAAACCGGC
TCGAGGCCTTGAAAGGCGACCGGCGGGGTCAGTTCAGCATTCGAATCAACGACCAGTGGCGGATCTGTTTTCGGTGGTCC
GCCGCGGGGCCAGAGGAGGTTGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT177748 WP_032413443.1 NZ_CP063216:25229-25672 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT177748 NZ_CP083648:c1863065-1862805 [Corynebacterium coyleae]
ATGGAGGACTTTCTCAAGGAAATGGAGATCACCCAGCACAAGCTGGCCGTCTCCATCGGCGTTCCGCCCCGCCGGATCAA
CGAAATTGTTCACGGTAAGCGCGCTGTAACCGCTGACACTGCCCTTCGTTTGGCGAAGTTTTTCGGGATGAGCCCCCAGT
TCTGGCTTGGGTTACAGGCCCAGTACGACCTGGATGTGGCAGAAGACAAAATCCTGGCGGAAATCGAACGGATTCAGCCG
CTCCAGGCTGCCTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4