Detailed information of TA system TA177717

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2313893..2314728
• Replicon: chromosome
• Accession: NZ_CP063214
• Organism: Escherichia coli strain ERP001

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: IL987_RS11320
• Protein ID: WP_001094445.1
• Coordinates: 2314351..2314728 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IL987_RS11315
• Protein ID: WP_024174331.1
• Coordinates: 2313893..2314261 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL987_RS11290	2311315..2311551	+	237	WP_193579432.1	DUF905 family protein	-
IL987_RS11295	2311642..2312460	+	819	WP_001234749.1	DUF945 domain-containing protein	-
IL987_RS11300	2312552..2313037	+	486	WP_000214415.1	antirestriction protein	-
IL987_RS11305	2313053..2313529	+	477	WP_193579415.1	RadC family protein	-
IL987_RS11310	2313598..2313819	+	222	WP_000692346.1	DUF987 domain-containing protein	-
IL987_RS11315	2313893..2314261	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IL987_RS11320	2314351..2314728	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
IL987_RS11325	2314725..2315213	+	489	WP_000761718.1	hypothetical protein	-
IL987_RS11330	2315225..2315422	+	198	WP_000838536.1	DUF957 domain-containing protein	-
IL987_RS11335	2315507..2316349	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
IL987_RS11340	2316691..2316861	+	171	Protein_2223	IS110 family transposase	-
IL987_RS11345	2317672..2318655	+	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T177717 WP_001094445.1 NZ_CP063214:2314351-2314728 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T177717 NZ_CP083638:2999683-3000147 [Escherichia fergusonii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATACTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT177717 WP_024174331.1 NZ_CP063214:2313893-2314261 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT177717 NZ_CP083638:2999348-2999683 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGATTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6