Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1215898..1216448 Replicon chromosome
Accession NZ_CP063046
Organism Escherichia coli strain KC-Dl-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag IL980_RS05810 Protein ID WP_001160966.1
Coordinates 1215898..1216212 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag IL980_RS05815 Protein ID WP_000125566.1
Coordinates 1216215..1216448 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL980_RS05785 1211573..1212859 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
IL980_RS05790 1212859..1213848 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IL980_RS05795 1213845..1214666 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IL980_RS05800 1214669..1215046 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IL980_RS05805 1215053..1215901 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IL980_RS05810 1215898..1216212 - 315 WP_001160966.1 CcdB family protein Toxin
IL980_RS05815 1216215..1216448 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IL980_RS05820 1216534..1217013 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
IL980_RS05825 1217205..1219067 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
IL980_RS05830 1219060..1219590 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IL980_RS05835 1219698..1221029 - 1332 WP_001183207.1 MFS transporter -
IL980_RS05840 1221088..1221375 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T177537 WP_001160966.1 NZ_CP063046:c1216212-1215898 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T177537 NZ_CP083530:c896644-896237 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT177537 WP_000125566.1 NZ_CP063046:c1216448-1216215 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT177537 NZ_CP083530:c896891-896625 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References