Detailed information of TA system TA177355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1669131..1670011
• Replicon: chromosome
• Accession: NZ_CP062949
• Organism: Bifidobacterium longum subsp. longum strain JCM 7050

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BL7050_RS07015
• Protein ID: WP_115784384.1
• Coordinates: 1669131..1669622 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8APN1
• Locus tag: BL7050_RS07020
• Protein ID: WP_007057212.1
• Coordinates: 1669619..1670011 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL7050_RS06995	1664203..1665435	+	1233	WP_193021245.1	sensor histidine kinase	-
BL7050_RS07000	1665470..1666195	+	726	WP_007056206.1	response regulator transcription factor	-
BL7050_RS07005	1666235..1666876	+	642	WP_007052627.1	GNAT family N-acetyltransferase	-
BL7050_RS07010	1666966..1669071	+	2106	WP_032747025.1	DUF262 domain-containing protein	-
BL7050_RS07015	1669131..1669622	-	492	WP_115784384.1	type II toxin-antitoxin system VapC family toxin	Toxin
BL7050_RS07020	1669619..1670011	-	393	WP_007057212.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BL7050_RS07025	1670156..1672855	-	2700	WP_100985607.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-146)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-61)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18589.73 Da
• Isoelectric Point: 5.7324

>T177355 WP_115784384.1 NZ_CP062949:c1669622-1669131 [Bifidobacterium longum subsp. longum]
MRQSKQVSILCDTNILIYYMLCTPRFIKDVMKLFERCVEREVKLSCASLSLKDVFYITPERFKERFMNDESMVVATTAKL
ILPEVAWSGISKLKELMTIVPIGAEECELAQSLRHAHNDFEDDLIIAAAQQSGATYVVTYDKKLAKHFPEYCKTPAEIVA
ALE

Nucleotide

• Length: 492 bp

>T177355 NZ_CP083449:65760-66014 [Pantoea eucrina]
GTGAAACTGACATGGTCTGCTGAAGCATGGGAAGATTATCTTTACTGGCAGGATACGGATAAAAAAACGGTAAAAAAAAT
AAATGAGCTCATTAAGGATGCCAGCAGGACGCCATTTGAGGGGAAGGGAAAGCCCGAACCTCTAAAGCATCATCTTGCGG
GGTTCTGGTCCCGGAGGATCACAGCTGAACATCGGCTTGTCTACGCCGTTAGCGATGATGCTCTGCTGATAGCATCCTGC
CGCTATCACTACTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15147.29 Da
• Isoelectric Point: 5.8624

>AT177355 WP_007057212.1 NZ_CP062949:c1670011-1669619 [Bifidobacterium longum subsp. longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREAEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDEIADERLKEYRHQSDYARYLEKERSLS

Nucleotide

• Length: 393 bp

>AT177355 NZ_CP083449:65512-65763 [Pantoea eucrina]
ATGAAAGCAATAAGCTACAGTGAAGCAAGACAGAATCTGTCTGCAACAATGCTGCAAACGGTTGAGGACAGAGCCCCGGT
GCTTATTACCCGTCAGAACGGTGAATCATGCGTATTGATGTCACTTGAGGAGTACAACTCACTTGAAGAAACTGCATACC
TGCTTCGGTCTCCGAAAAATGCGCGCAGACTTATGGACTCCATTGAAAGCCTTAAAAGCGGTAACGGTATGGAGAGGGAC
GTCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: F8APN1