Detailed information of TA system TA177102

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115753..116022
• Replicon: plasmid unnamednovel_1
• Accession: NZ_CP062879
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IMP69_RS24850
• Protein ID: WP_001312861.1
• Coordinates: 115864..116022 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	115753..115818 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24820	111518..112045	+	528	WP_000290819.1	single-stranded DNA-binding protein	-
IMP69_RS24825	112103..112336	+	234	WP_000006010.1	DUF905 family protein	-
IMP69_RS24830	112401..114365	+	1965	WP_195686730.1	ParB/RepB/Spo0J family partition protein	-
IMP69_RS24835	114434..114868	+	435	WP_000845948.1	conjugation system SOS inhibitor PsiB	-
IMP69_RS24840	114865..115584	+	720	WP_001276273.1	plasmid SOS inhibition protein A	-
-	115596..115820	+	225	NuclAT_0	-	-
-	115596..115820	+	225	NuclAT_0	-	-
-	115596..115820	+	225	NuclAT_0	-	-
-	115596..115820	+	225	NuclAT_0	-	-
IMP69_RS24845	115605..115784	-	180	WP_001309233.1	hypothetical protein	-
-	115753..115818	-	66	-	-	Antitoxin
IMP69_RS24850	115864..116022	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IMP69_RS24855	116939..117226	+	288	WP_000107526.1	hypothetical protein	-
IMP69_RS24860	117382..118079	-	698	Protein_122	IS1 family transposase	-
IMP69_RS24865	118137..118637	+	501	WP_187524466.1	hypothetical protein	-
IMP69_RS24870	118688..120895	+	2208	WP_195686731.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..131388	131388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T177102 WP_001312861.1 NZ_CP062879:115864-116022 [Escherichia coli O157:H16]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T177102 NZ_CP083101:c2473776-2473039 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT177102 NZ_CP062879:c115818-115753 [Escherichia coli O157:H16]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin