Detailed information of TA system TA177084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2228381..2228931
• Replicon: chromosome
• Accession: NZ_CP062876
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IMP69_RS10995
• Protein ID: WP_195686700.1
• Coordinates: 2228381..2228695 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: IMP69_RS11000
• Protein ID: WP_000125566.1
• Coordinates: 2228698..2228931 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS10970	2224056..2225342	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
IMP69_RS10975	2225342..2226331	+	990	WP_000241253.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IMP69_RS10980	2226328..2227149	+	822	WP_001065377.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IMP69_RS10985	2227152..2227529	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IMP69_RS10990	2227536..2228384	+	849	WP_000257189.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IMP69_RS10995	2228381..2228695	-	315	WP_195686700.1	CcdB family protein	Toxin
IMP69_RS11000	2228698..2228931	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IMP69_RS11005	2229017..2229496	-	480	WP_001487721.1	type 3 dihydrofolate reductase	-
IMP69_RS11010	2229688..2231550	-	1863	WP_195686701.1	glutathione-regulated potassium-efflux system protein KefC	-
IMP69_RS11015	2231543..2232073	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IMP69_RS11020	2232184..2233515	-	1332	Protein_2155	MFS transporter	-
IMP69_RS11025	2233573..2233860	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.77 Da
• Isoelectric Point: 9.0851

>T177084 WP_195686700.1 NZ_CP062876:c2228695-2228381 [Escherichia coli O157:H16]
MQFTVYRSRSRNATFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T177084 NZ_CP083071:c105462-104980 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT177084 WP_000125566.1 NZ_CP062876:c2228931-2228698 [Escherichia coli O157:H16]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT177084 NZ_CP083071:c105731-105453 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9