Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3448323..3449160 Replicon chromosome
Accession NZ_CP062868
Organism Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IMP84_RS16540 Protein ID WP_000227784.1
Coordinates 3448618..3449160 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IMP84_RS16535 Protein ID WP_001297137.1
Coordinates 3448323..3448634 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP84_RS16510 3443343..3444290 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
IMP84_RS16515 3444312..3446303 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IMP84_RS16520 3446293..3446907 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IMP84_RS16525 3446907..3447236 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IMP84_RS16530 3447248..3448138 + 891 WP_000971336.1 heme o synthase -
IMP84_RS16535 3448323..3448634 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IMP84_RS16540 3448618..3449160 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IMP84_RS16545 3449216..3450151 - 936 WP_001365761.1 sel1 repeat family protein -
IMP84_RS16550 3450559..3451923 + 1365 WP_001000974.1 MFS transporter -
IMP84_RS16555 3452051..3452542 - 492 WP_001138904.1 nucleotide binding protein YajQ -
IMP84_RS16560 3452710..3453621 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T177016 WP_000227784.1 NZ_CP062868:3448618-3449160 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T177016 NZ_CP083047:c5351722-5351339 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT177016 WP_001297137.1 NZ_CP062868:3448323-3448634 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT177016 NZ_CP083047:c5351964-5351722 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References