Detailed information of TA system TA176986

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62709..62963
• Replicon: plasmid unnamed972
• Accession: NZ_CP062867
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IMP90_RS23790
• Protein ID: WP_001553767.1
• Coordinates: 62709..62915 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	62902..62963 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS23760	58015..59228	+	1214	WP_162841971.1	IS3 family transposase	-
IMP90_RS23765	59505..59792	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IMP90_RS23770	59789..60040	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IMP90_RS23775	61006..61863	-	858	WP_000131010.1	incFII family plasmid replication initiator RepA	-
IMP90_RS23780	61856..61930	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMP90_RS23785	62177..62425	-	249	WP_000083835.1	replication regulatory protein RepA	-
IMP90_RS23790	62709..62915	-	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	62902..62963	+	62	NuclAT_2	-	Antitoxin
-	62902..62963	+	62	NuclAT_2	-	Antitoxin
-	62902..62963	+	62	NuclAT_2	-	Antitoxin
-	62902..62963	+	62	NuclAT_2	-	Antitoxin
IMP90_RS23795	63138..63520	-	383	Protein_56	hypothetical protein	-
IMP90_RS23800	63623..64084	-	462	WP_000760078.1	thermonuclease family protein	-
IMP90_RS23805	64565..65242	+	678	WP_001682408.1	IS66-like element accessory protein TnpA	-
IMP90_RS23810	65242..65589	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
IMP90_RS23815	65609..67180	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA	1..155756	155756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7804.35 Da
• Isoelectric Point: 8.8807

>T176986 WP_001553767.1 NZ_CP062867:c62915-62709 [Escherichia coli O39:H21]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T176986 NZ_CP083036:c2771287-2769965 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCTACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT176986 NZ_CP062867:62902-62963 [Escherichia coli O39:H21]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin