Detailed information of TA system TA176719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 746912..747550
• Replicon: chromosome
• Accession: NZ_CP062824
• Organism: Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: IMQ31_RS03755
• Protein ID: WP_000813797.1
• Coordinates: 747374..747550 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMQ31_RS03750
• Protein ID: WP_076611057.1
• Coordinates: 746912..747328 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ31_RS03730	742064..743005	-	942	WP_001251315.1	ABC transporter permease	-
IMQ31_RS03735	743006..744019	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
IMQ31_RS03740	744037..745182	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
IMQ31_RS03745	745427..746833	-	1407	WP_024229532.1	PLP-dependent aminotransferase family protein	-
IMQ31_RS03750	746912..747328	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IMQ31_RS03755	747374..747550	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IMQ31_RS03760	747772..748002	+	231	WP_000494244.1	YncJ family protein	-
IMQ31_RS03765	748094..750055	-	1962	WP_024229530.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IMQ31_RS03770	750128..750664	-	537	WP_024229529.1	DNA-binding transcriptional regulator SutR	-
IMQ31_RS03775	750717..751928	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T176719 WP_000813797.1 NZ_CP062824:c747550-747374 [Escherichia coli O167:H26]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176719 NZ_CP082916:c1764996-1764505 [Salmonella enterica subsp. enterica serovar Newlands]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT176719 WP_076611057.1 NZ_CP062824:c747328-746912 [Escherichia coli O167:H26]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176719 NZ_CP082916:c1765286-1764993 [Salmonella enterica subsp. enterica serovar Newlands]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A829J715