Detailed information of TA system TA176364

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3053273..3053498
• Replicon: chromosome
• Accession: NZ_CP062766
• Organism: Escherichia coli O157:H7 strain Z866

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ34_RS15435
• Protein ID: WP_000813258.1
• Coordinates: 3053273..3053428 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3053440..3053498 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ34_RS15385	3048276..3048707	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ34_RS15400	3049158..3049871	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ34_RS15405	3050007..3050204	-	198	WP_000917763.1	hypothetical protein	-
ILQ34_RS15410	3050429..3050983	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ34_RS15415	3051046..3051351	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ34_RS15420	3051364..3052413	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ34_RS15425	3052415..3052687	-	273	WP_000191872.1	hypothetical protein	-
ILQ34_RS15430	3052809..3053153	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ34_RS15435	3053273..3053428	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3053440..3053498	+	59	-	-	Antitoxin
ILQ34_RS15440	3053719..3054276	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ34_RS15445	3054278..3054496	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ34_RS15450	3054624..3054935	-	312	WP_001289673.1	hypothetical protein	-
ILQ34_RS15455	3054928..3055155	-	228	WP_000699809.1	hypothetical protein	-
ILQ34_RS15460	3055152..3055433	-	282	WP_000603384.1	DNA-binding protein	-
ILQ34_RS15465	3055466..3056182	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ34_RS15470	3056216..3056677	-	462	WP_000139447.1	replication protein	-
ILQ34_RS15475	3056670..3057713	-	1044	WP_001262402.1	hypothetical protein	-
ILQ34_RS15480	3057782..3058207	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ34_RS15485	3058191..3058433	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7' / galU	2970359..3137513	167154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176364 WP_000813258.1 NZ_CP062766:c3053428-3053273 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176364 NZ_CP082791:c1902165-1901848 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176364 NZ_CP062766:3053440-3053498 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin