Detailed information of TA system TA176319

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2356128..2356353
• Replicon: chromosome
• Accession: NZ_CP062763
• Organism: Escherichia coli O157:H7 strain Z885

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ36_RS11970
• Protein ID: WP_000813258.1
• Coordinates: 2356198..2356353 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2356128..2356186 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ36_RS11920	2351193..2351435	+	243	WP_000747948.1	hypothetical protein	-
ILQ36_RS11925	2351419..2351844	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ36_RS11930	2351913..2352956	+	1044	WP_001262402.1	hypothetical protein	-
ILQ36_RS11935	2352949..2353410	+	462	WP_000139447.1	replication protein	-
ILQ36_RS11940	2353444..2354160	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ36_RS11945	2354193..2354474	+	282	WP_000603384.1	DNA-binding protein	-
ILQ36_RS11950	2354471..2354698	+	228	WP_000699809.1	hypothetical protein	-
ILQ36_RS11955	2354691..2355002	+	312	WP_001289673.1	hypothetical protein	-
ILQ36_RS11960	2355130..2355348	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ36_RS11965	2355350..2355907	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2356128..2356186	-	59	-	-	Antitoxin
ILQ36_RS11970	2356198..2356353	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ36_RS11975	2356473..2356817	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ36_RS11980	2356939..2357211	+	273	WP_000191872.1	hypothetical protein	-
ILQ36_RS11985	2357213..2358262	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ36_RS11990	2358275..2358580	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ36_RS11995	2358643..2359197	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ36_RS12000	2359422..2359619	+	198	WP_000917763.1	hypothetical protein	-
ILQ36_RS12005	2359755..2360468	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ36_RS12020	2360919..2361350	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	galU / paa / nleG7' / nleC	2225118..2411143	186025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176319 WP_000813258.1 NZ_CP062763:2356198-2356353 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176319 NZ_CP082777:c3179715-3179461 [Escherichia coli O25:H1]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176319 NZ_CP062763:c2356186-2356128 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin