Detailed information of TA system TA176064

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3066580..3066805
• Replicon: chromosome
• Accession: NZ_CP062744
• Organism: Escherichia coli O157:H7 strain Z1615

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ43_RS15595
• Protein ID: WP_000813258.1
• Coordinates: 3066580..3066735 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3066747..3066805 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ43_RS15545	3061583..3062014	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ43_RS15560	3062465..3063178	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ43_RS15565	3063314..3063511	-	198	WP_000917763.1	hypothetical protein	-
ILQ43_RS15570	3063736..3064290	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ43_RS15575	3064353..3064658	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ43_RS15580	3064671..3065720	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ43_RS15585	3065722..3065994	-	273	WP_000191872.1	hypothetical protein	-
ILQ43_RS15590	3066116..3066460	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ43_RS15595	3066580..3066735	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3066747..3066805	+	59	-	-	Antitoxin
ILQ43_RS15600	3067026..3067583	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ43_RS15605	3067585..3067803	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ43_RS15610	3067931..3068242	-	312	WP_001289673.1	hypothetical protein	-
ILQ43_RS15615	3068235..3068462	-	228	WP_000699809.1	hypothetical protein	-
ILQ43_RS15620	3068459..3068740	-	282	WP_000603384.1	DNA-binding protein	-
ILQ43_RS15625	3068773..3069489	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ43_RS15630	3069523..3069984	-	462	WP_000139447.1	replication protein	-
ILQ43_RS15635	3069977..3071020	-	1044	WP_001262402.1	hypothetical protein	-
ILQ43_RS15640	3071089..3071514	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ43_RS15645	3071498..3071740	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7' / paa	2983664..3128480	144816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176064 WP_000813258.1 NZ_CP062744:c3066735-3066580 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176064 NZ_CP082349:2321160-2321447 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176064 NZ_CP062744:3066747-3066805 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin