Detailed information of TA system TA175915

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2673215..2673440
• Replicon: chromosome
• Accession: NZ_CP062733
• Organism: Escherichia coli O157:H7 strain Z1767

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ47_RS13440
• Protein ID: WP_000813258.1
• Coordinates: 2673215..2673370 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2673382..2673440 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ47_RS13390	2668219..2668650	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ47_RS13405	2669101..2669814	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ47_RS13410	2669949..2670146	-	198	WP_000917763.1	hypothetical protein	-
ILQ47_RS13415	2670371..2670925	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ47_RS13420	2670988..2671293	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ47_RS13425	2671306..2672355	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ47_RS13430	2672357..2672629	-	273	WP_000191872.1	hypothetical protein	-
ILQ47_RS13435	2672751..2673095	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ47_RS13440	2673215..2673370	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2673382..2673440	+	59	-	-	Antitoxin
ILQ47_RS13445	2673661..2674218	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ47_RS13450	2674220..2674438	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ47_RS13455	2674566..2674877	-	312	WP_001289673.1	hypothetical protein	-
ILQ47_RS13460	2674870..2675097	-	228	WP_000699809.1	hypothetical protein	-
ILQ47_RS13465	2675094..2675375	-	282	WP_000603384.1	DNA-binding protein	-
ILQ47_RS13470	2675408..2676124	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ47_RS13475	2676158..2676619	-	462	WP_000139447.1	replication protein	-
ILQ47_RS13480	2676612..2677655	-	1044	WP_001262402.1	hypothetical protein	-
ILQ47_RS13485	2677724..2678149	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ47_RS13490	2678133..2678375	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7' / paa	2590300..2742834	152534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175915 WP_000813258.1 NZ_CP062733:c2673370-2673215 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175915 NZ_CP082269:50685-51260 [Enterobacter sp. C2]
ATGAGGCACTCGCCATATCCGGTAGTATTAGATGCATGTGTACTTTATCCTGCAAGGCTCAGGGATCTACTGATGCATCT
TGGCCTTGTAGGCTTATACCAACCGAAATGGAGTAGAGATATACACCATGAGTGGTGTAGAAATTTGTTGAAAAACAGAT
CGGACATCCAGGCAGAAACGCTTGAGTACACCGTTAAATTGATGAATCAGGCGCTACCTGATGCAAATATCACTGGTTAT
GAGTATTTAATTGAAGGGTTAACGCTTCCAGATATTGATGACCGACATGTCCTTGCAGCGGCTATTCGTGCAAAAGCAGA
AGTTATTGTCACACTGAATAAAAAGGATTTTCCCGCTGTTTTACTTGCTCCATTCAATATCGAAGCTCTTCATCCCGATG
AGTTTATCTCTGATCTGTTCGATCTTAATCATGCTTTGGCGCTTGAAGCGGTGAGAAGGCAGCGGCAGTCTCTCAGACAT
CCTCCGATGGCTGTAGAAGATTTTTTGGCAATGTTATTAAAGCAAGGGTTAACGAGAACGGTTAAGGTTTTAGAAGGGTA
TGAATGTATTCTTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175915 NZ_CP062733:2673382-2673440 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin