Detailed information of TA system TA175888

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3572717..3572942
• Replicon: chromosome
• Accession: NZ_CP062731
• Organism: Escherichia coli O157:H7 strain Z1768

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ48_RS18350
• Protein ID: WP_000813263.1
• Coordinates: 3572717..3572872 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3572884..3572942 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ48_RS18315	3568171..3568884	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ48_RS18320	3569022..3569218	-	197	Protein_3585	TrmB family transcriptional regulator	-
ILQ48_RS18325	3569505..3570323	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ48_RS18330	3570475..3570846	-	372	WP_000090264.1	antitermination protein	-
ILQ48_RS18335	3570836..3571207	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ48_RS18340	3571220..3572269	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ48_RS18345	3572271..3572549	-	279	WP_001341388.1	hypothetical protein	-
ILQ48_RS18350	3572717..3572872	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3572884..3572942	+	59	-	-	Antitoxin
ILQ48_RS18355	3573477..3574250	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ48_RS18360	3574602..3575015	-	414	WP_001151233.1	DUF977 family protein	-
ILQ48_RS18365	3575031..3575801	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ48_RS18370	3575823..3576569	-	747	WP_000788751.1	ATP-binding protein	-
ILQ48_RS18375	3576576..3577667	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175888 WP_000813263.1 NZ_CP062731:c3572872-3572717 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175888 NZ_CP082236:5625764-5626057 [Pseudomonas sp. SC3(2021)]
ATGGTTTCGAGGCCGATTGAGTGGAGGCCCGAAGCGCGGGCGAAACTGGTAACAGTGCTCGAATACATAGTCGAAAGGAA
TGTTGTAGCAGCCAAGGAGATCTTGAAGGCAGTTGGAAAAGCCATAGCTTATCTTCCTGATCATCCCTATCTCTATCGAT
CAGGCCGGATCACCGGCACTCGGGAGATCGTTGTCAATTCCAGCTATCTGATCATCTACCGAGTGACGGATCGGATCGAG
ATCGTTAACGTTCTGCATGCGCGTCAGGAGTATCCGCGACGCGATTCTTTCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175888 NZ_CP062731:3572884-3572942 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin